define the target population in research

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• What is target population: Definition & examples

Researchers should consider many factors for the research to be successful when conducting research. One of the essential steps is identifying the target population for the research study early on while planning a market research study and thinking about goals and objectives . 

A target population sets a clear direction on the scope and object of the research and data types. This article will explain what the target population is in a research example and Frequently ask questions about the target population with all the details. 

• What is the target population in research?

The population that the intervention is intended to study and take conclusions from is known as the target population. A target population, also referred to as a target audience , is a group of people with particular characteristics that may be effectively defined to distinguish them from the general population. 

The target population aims to comprehend and assess their preferences and behaviors to promote a particular good or service or to research a specific feature that frequently manifests itself in their behavior, such as behavior patterns. It is a notion that has to do with business market segmentation tactics. 

A target population is typically a group or collection of factors you want to learn more about. The target population is a subset of the general public identified as the targeted market for a given product, advertising, or research. It is a subset of the entire population chosen to serve as the objective audience.

• How to determine the target population in a research design

Identifying the target population requires careful preparation , precise research questions, and a robust study design . It will assist you in obtaining trustworthy and accurate information that responds to your study issue or concern.

The target group is frequently chosen based on characteristics or demographics such as age , gender , employment , income , or health condition . The research's conclusions are then extrapolated to the broader population from whom the target sample was selected. 

How to choose your target population

Narrowing objectives is one of the most sensible things for businesses to do when identifying a target market so that their goods or services may be promoted effectively. The quality of the service will rise, and controllability will be significantly aided by an attempt to offer a more specific target population. 

First, you should define the target population you wish to attract with your marketing campaigns to create a target audience. The following steps will assist you in determining the population. 

• Set business or brand goals: This is essential in creating a content strategy or developing a marketing plan. 
• Define your study’s objective: You should clearly state your study's motivation and objective.
• Determine the population features: Determine the features of your intended audience. Gender, age, educational background, socio-economic situation, and career should be determined.
• Market research: Market research is the process of collecting and analyzing data about the market in which a business operates to understand the target audience and the potential of a specific product or service. 
• 3 target population examples

Target population determination is an ongoing process that requires research, analysis, and adjustments. You can identify the specific group of people you want to reach with your marketing efforts and create a strategy to interact with them effectively. Here are 3 target population examples. 

• Determining target population according to specific features: Let's say you are researching school service fees. First, you should consider specific characteristics when determining the target population. As parents whose children go to school and parents who send their children to school by service, you can narrow down your target audience in your research. 
• Determining target population according to characteristic features: Another example is you are launching a new perfume as a cosmetics company. Your further perfume appeals to young women. You should determine the age group of the target population . Then you focus on gender , and maybe you can consider the women using perfume. You can choose your target population in this way. 
• Determining target population according to target audience: The last example is that you are a business manager and want to measure the customer's satisfaction with a product. The target population would be all the customers of a particular business. 
• Frequently asked questions about the target population

The target population is a big group of people that researchers are looking at. The target population is very similar to other population types. There are frequently asked questions about the target population.

What is the target population in quantitative research

In quantitative research, the term "target population" refers to the group of people or things the researcher wishes to analyze and draw conclusions about based on the data collected.

What is the target population in qualitative research

In qualitative research, the term "target population" refers to the group of participants or subjects who are of interest to the researcher to explore, comprehend, and examine subjective experiences, behaviors, attitudes, or occurrences. Audiences often choose The target group based on criteria like gender, age, education, and other features.

Target population vs. sample population

The difference between the sample population and the target population is that the target population is a more significant subset of the target population chosen for the research project. In contrast, the sample population is the entire group of people or things the researcher is interested in working with.

Target population vs. accessible population

The difference between the target and accessible population is that an accessible population is that portion of the target population that may theoretically be included in the study. In contrast, a target population is the whole set of instances the researcher desires to analyze.

Target population vs. sampling frame

The difference between the target population and the sampling frame is that the sampling frame is a subset of the target population. The sampling frame typically serves as the starting point for the sampling process. The sample is a portion of the population and the individuals or items you have access to. In contrast, the target population is the entire group of people or objects to which you desire to generalize the results of your study.

Target population vs. sample

The difference between the population and the sample is the target population is much larger than the sample. The target population is the entire group or items the researcher intends to examine and draw conclusions about. On the other hand, a sample is a subset of the target population that is selected for study.

In conclusion, a researcher's target population is the group they are trying to comprehend. When the target audience is analyzed, new information may be found that enables the business to launch various advertising campaigns tailored to the target population's income levels and attitudes. 

This article has explained the definition of the target population, How to determine the target population in a research design, and examples of the target population. When you read this article, you can learn all details about the target population.

Sena is a content writer at forms.app. She likes to read and write articles on different topics. Sena also likes to learn about different cultures and travel. She likes to study and learn different languages. Her specialty is linguistics, surveys, survey questions, and sampling methods.

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Unraveling Research Population and Sample: Understanding their role in statistical inference

Research population and sample serve as the cornerstones of any scientific inquiry. They hold the power to unlock the mysteries hidden within data. Understanding the dynamics between the research population and sample is crucial for researchers. It ensures the validity, reliability, and generalizability of their findings. In this article, we uncover the profound role of the research population and sample, unveiling their differences and importance that reshapes our understanding of complex phenomena. Ultimately, this empowers researchers to make informed conclusions and drive meaningful advancements in our respective fields.

Table of Contents

What Is Population?

The research population, also known as the target population, refers to the entire group or set of individuals, objects, or events that possess specific characteristics and are of interest to the researcher. It represents the larger population from which a sample is drawn. The research population is defined based on the research objectives and the specific parameters or attributes under investigation. For example, in a study on the effects of a new drug, the research population would encompass all individuals who could potentially benefit from or be affected by the medication.

When Is Data Collection From a Population Preferred?

In certain scenarios where a comprehensive understanding of the entire group is required, it becomes necessary to collect data from a population. Here are a few situations when one prefers to collect data from a population:

1. Small or Accessible Population

When the research population is small or easily accessible, it may be feasible to collect data from the entire population. This is often the case in studies conducted within specific organizations, small communities, or well-defined groups where the population size is manageable.

2. Census or Complete Enumeration

In some cases, such as government surveys or official statistics, a census or complete enumeration of the population is necessary. This approach aims to gather data from every individual or entity within the population. This is typically done to ensure accurate representation and eliminate sampling errors.

3. Unique or Critical Characteristics

If the research focuses on a specific characteristic or trait that is rare and critical to the study, collecting data from the entire population may be necessary. This could be the case in studies related to rare diseases, endangered species, or specific genetic markers.

4. Legal or Regulatory Requirements

Certain legal or regulatory frameworks may require data collection from the entire population. For instance, government agencies might need comprehensive data on income levels, demographic characteristics, or healthcare utilization for policy-making or resource allocation purposes.

5. Precision or Accuracy Requirements

In situations where a high level of precision or accuracy is necessary, researchers may opt for population-level data collection. By doing so, they mitigate the potential for sampling error and obtain more reliable estimates of population parameters.

What Is a Sample?

A sample is a subset of the research population that is carefully selected to represent its characteristics. Researchers study this smaller, manageable group to draw inferences that they can generalize to the larger population. The selection of the sample must be conducted in a manner that ensures it accurately reflects the diversity and pertinent attributes of the research population. By studying a sample, researchers can gather data more efficiently and cost-effectively compared to studying the entire population. The findings from the sample are then extrapolated to make conclusions about the larger research population.

What Is Sampling and Why Is It Important?

Sampling refers to the process of selecting a sample from a larger group or population of interest in order to gather data and make inferences. The goal of sampling is to obtain a sample that is representative of the population, meaning that the sample accurately reflects the key attributes, variations, and proportions present in the population. By studying the sample, researchers can draw conclusions or make predictions about the larger population with a certain level of confidence.

Collecting data from a sample, rather than the entire population, offers several advantages and is often necessary due to practical constraints. Here are some reasons to collect data from a sample:

1. Cost and Resource Efficiency

Collecting data from an entire population can be expensive and time-consuming. Sampling allows researchers to gather information from a smaller subset of the population, reducing costs and resource requirements. It is often more practical and feasible to collect data from a sample, especially when the population size is large or geographically dispersed.

2. Time Constraints

Conducting research with a sample allows for quicker data collection and analysis compared to studying the entire population. It saves time by focusing efforts on a smaller group, enabling researchers to obtain results more efficiently. This is particularly beneficial in time-sensitive research projects or situations that necessitate prompt decision-making.

3. Manageable Data Collection

Working with a sample makes data collection more manageable . Researchers can concentrate their efforts on a smaller group, allowing for more detailed and thorough data collection methods. Furthermore, it is more convenient and reliable to store and conduct statistical analyses on smaller datasets. This also facilitates in-depth insights and a more comprehensive understanding of the research topic.

4. Statistical Inference

Collecting data from a well-selected and representative sample enables valid statistical inference. By using appropriate statistical techniques, researchers can generalize the findings from the sample to the larger population. This allows for meaningful inferences, predictions, and estimation of population parameters, thus providing insights beyond the specific individuals or elements in the sample.

5. Ethical Considerations

In certain cases, collecting data from an entire population may pose ethical challenges, such as invasion of privacy or burdening participants. Sampling helps protect the privacy and well-being of individuals by reducing the burden of data collection. It allows researchers to obtain valuable information while ensuring ethical standards are maintained .

Key Steps Involved in the Sampling Process

Sampling is a valuable tool in research; however, it is important to carefully consider the sampling method, sample size, and potential biases to ensure that the findings accurately represent the larger population and are valid for making conclusions and generalizations. While the specific steps may vary depending on the research context, here is a general outline of the sampling process:

1. Define the Population

Clearly define the target population for your research study. The population should encompass the group of individuals, elements, or units that you want to draw conclusions about.

2. Define the Sampling Frame

Create a sampling frame, which is a list or representation of the individuals or elements in the target population. The sampling frame should be comprehensive and accurately reflect the population you want to study.

3. Determine the Sampling Method

Select an appropriate sampling method based on your research objectives, available resources, and the characteristics of the population. You can perform sampling by either utilizing probability-based or non-probability-based techniques. Common sampling methods include random sampling, stratified sampling, cluster sampling, and convenience sampling.

4. Determine Sample Size

Determine the desired sample size based on statistical considerations, such as the level of precision required, desired confidence level, and expected variability within the population. Larger sample sizes generally reduce sampling error but may be constrained by practical limitations.

5. Collect Data

Once the sample is selected using the appropriate technique, collect the necessary data according to the research design and data collection methods . Ensure that you use standardized and consistent data collection process that is also appropriate for your research objectives.

6. Analyze the Data

Perform the necessary statistical analyses on the collected data to derive meaningful insights. Use appropriate statistical techniques to make inferences, estimate population parameters, test hypotheses, or identify patterns and relationships within the data.

Population vs Sample — Differences and examples

While the population provides a comprehensive overview of the entire group under study, the sample, on the other hand, allows researchers to draw inferences and make generalizations about the population. Researchers should employ careful sampling techniques to ensure that the sample is representative and accurately reflects the characteristics and variability of the population.

Research Study: Investigating the prevalence of stress among high school students in a specific city and its impact on academic performance.

Population: All high school students in a particular city

Sampling Frame: The sampling frame would involve obtaining a comprehensive list of all high schools in the specific city. A random selection of schools would be made from this list to ensure representation from different areas and demographics of the city.

Sample: Randomly selected 500 high school students from different schools in the city

The sample represents a subset of the entire population of high school students in the city.

Research Study: Assessing the effectiveness of a new medication in managing symptoms and improving quality of life in patients with the specific medical condition.

Population: Patients diagnosed with a specific medical condition

Sampling Frame: The sampling frame for this study would involve accessing medical records or databases that include information on patients diagnosed with the specific medical condition. Researchers would select a convenient sample of patients who meet the inclusion criteria from the sampling frame.

Sample: Convenient sample of 100 patients from a local clinic who meet the inclusion criteria for the study

The sample consists of patients from the larger population of individuals diagnosed with the medical condition.

Research Study: Investigating community perceptions of safety and satisfaction with local amenities in the neighborhood.

Population: Residents of a specific neighborhood

Sampling Frame: The sampling frame for this study would involve obtaining a list of residential addresses within the specific neighborhood. Various sources such as census data, voter registration records, or community databases offer the means to obtain this information. From the sampling frame, researchers would randomly select a cluster sample of households to ensure representation from different areas within the neighborhood.

Sample: Cluster sample of 50 households randomly selected from different blocks within the neighborhood

The sample represents a subset of the entire population of residents living in the neighborhood.

To summarize, sampling allows for cost-effective data collection, easier statistical analysis, and increased practicality compared to studying the entire population. However, despite these advantages, sampling is subject to various challenges. These challenges include sampling bias, non-response bias, and the potential for sampling errors.

To minimize bias and enhance the validity of research findings , researchers should employ appropriate sampling techniques, clearly define the population, establish a comprehensive sampling frame, and monitor the sampling process for potential biases. Validating findings by comparing them to known population characteristics can also help evaluate the generalizability of the results. Properly understanding and implementing sampling techniques ensure that research findings are accurate, reliable, and representative of the larger population. By carefully considering the choice of population and sample, researchers can draw meaningful conclusions and, consequently, make valuable contributions to their respective fields of study.

Now, it’s your turn! Take a moment to think about a research question that interests you. Consider the population that would be relevant to your inquiry. Who would you include in your sample? How would you go about selecting them? Reflecting on these aspects will help you appreciate the intricacies involved in designing a research study. Let us know about it in the comment section below or reach out to us using  #AskEnago  and tag  @EnagoAcademy  on  Twitter ,  Facebook , and  Quora .

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What is a Target Population in Research?

• September 1, 2024

What is a Target Population?

Your study’s  target population is the entire group of individuals or elements to which you want to generalize (for quantitative research)  or potentially transfer your study findings (for qualitative research). It’s the complete set of units (people, objects, events) that share the characteristics of interest for the research question.

Role in the Research Process 

Target populations play several roles in the research process:

They provide the population from with you will pull your study sample.

They help define your research scope.

They support study generalization.

Consider a study on the effectiveness of a new reading program for elementary school students in a public school in the Northeast. In this example, your target population is all urban elementary school students (grades 1-5) in public schools in the Northeast.

The above parameters:

• Specify the age group (elementary school students)
• Define the educational setting (public schools)
• Establish the geographical boundary (Northeastern United States)

From this larger population you will pull a sample, such as students from randomly selected schools across the Northeast. In quantitative studies, the findings from this sample would then be used to make inferences about the effectiveness of the reading program for the entire target population of U.S. public elementary school students. In qualitative research, we don’t think in terms of generalizability. However, others who read your research will decide if your target population and sample are similar enough to their own for your findings to be transferable. 

A good rule of thumb is to remember that your target population is generally (much) larger than your sample. If you already know the characteristics of your sample, then consider which larger group that sample is a part of. Think bigger and broader. This larger group is likely population from which you will pull your sample.   

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Statistics without tears: Populations and samples

Amitav banerjee, suprakash chaudhury.

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Address for correspondence: Dr. Amitav Banerjee, Department of Community Medicine, D Y Patil Medical College, Pune – 18, India E-mail: [email protected]

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Research studies are usually carried out on sample of subjects rather than whole populations. The most challenging aspect of fieldwork is drawing a random sample from the target population to which the results of the study would be generalized. In actual practice, the task is so difficult that some sampling bias occurs in almost all studies to a lesser or greater degree. In order to assess the degree of this bias, the informed reader of medical literature should have some understanding of the population from which the sample was drawn. The ultimate decision on whether the results of a particular study can be generalized to a larger population depends on this understanding. The subsequent deliberations dwell on sampling strategies for different types of research and also a brief description of different sampling methods.

Keywords: Methods, population, sample

Research workers in the early 19th century endeavored to survey entire populations. This feat was tedious, and the research work suffered accordingly. Current researchers work only with a small portion of the whole population (a sample) from which they draw inferences about the population from which the sample was drawn.

This inferential leap or generalization from samples to population, a feature of inductive or empirical research, can be full of pitfalls. In clinical medicine, it is not sufficient merely to describe a patient without assessing the underlying condition by a detailed history and clinical examination. The signs and symptoms are then interpreted against the total background of the patient's history and clinical examination including mental state examination. Similarly, in inferential statistics, it is not enough to just describe the results in the sample. One has to critically appraise the real worth or representativeness of that particular sample. The following discussion endeavors to explain the inputs required for making a correct inference from a sample to the target population.

TARGET POPULATION

Any inferences from a sample refer only to the defined population from which the sample has been properly selected. We may call this the target population. For example, if in a sample of lawyers from Delhi High Court it is found that 5% are having alcohol dependence syndrome, can we say that 5% of all lawyers all over the world are alcoholics? Obviously not, as the lawyers of Delhi High Court may be an institution by themselves and may not represent the global lawyers′ community. The findings of this study, therefore, apply only to Delhi High Court lawyers from which a representative sample was taken. Of course, this finding may nevertheless be interesting, but only as a pointer to further research. The data on lawyers in a particular city tell us nothing about lawyers in other cities or countries.

POPULATIONS IN INFERENTIAL STATISTICS

In statistics, a population is an entire group about which some information is required to be ascertained. A statistical population need not consist only of people. We can have population of heights, weights, BMIs, hemoglobin levels, events, outcomes, so long as the population is well defined with explicit inclusion and exclusion criteria. In selecting a population for study, the research question or purpose of the study will suggest a suitable definition of the population to be studied, in terms of location and restriction to a particular age group, sex or occupation. The population must be fully defined so that those to be included and excluded are clearly spelt out (inclusion and exclusion criteria). For example, if we say that our study populations are all lawyers in Delhi, we should state whether those lawyers are included who have retired, are working part-time, or non-practicing, or those who have left the city but still registered at Delhi.

Use of the word population in epidemiological research does not correspond always with its demographic meaning of an entire group of people living within certain geographic or political boundaries. A population for a research study may comprise groups of people defined in many different ways, for example, coal mine workers in Dhanbad, children exposed to German measles during intrauterine life, or pilgrims traveling to Kumbh Mela at Allahabad.

GENERALIZATION (INFERENCES) FROM A POPULATION

When generalizing from observations made on a sample to a larger population, certain issues will dictate judgment. For example, generalizing from observations made on the mental health status of a sample of lawyers in Delhi to the mental health status of all lawyers in Delhi is a formalized procedure, in so far as the errors (sampling or random) which this may hazard can, to some extent, be calculated in advance. However, if we attempt to generalize further, for instance, about the mental statuses of all lawyers in the country as a whole, we hazard further pitfalls which cannot be specified in advance. We do not know to what extent the study sample and population of Delhi is typical of the larger population – that of the whole country – to which it belongs.

The dilemmas in defining populations differ for descriptive and analytic studies.

POPULATION IN DESCRIPTIVE STUDIES

In descriptive studies, it is customary to define a study population and then make observations on a sample taken from it. Study populations may be defined by geographic location, age, sex, with additional definitions of attributes and variables such as occupation, religion and ethnic group.[ 1 ]

Geographic location

In field studies, it may be desirable to use a population defined by an administrative boundary such as a district or a state. This may facilitate the co-operation of the local administrative authorities and the study participants. Moreover, basic demographic data on the population such as population size, age, gender distribution (needed for calculating age- and sex-specific rates) available from census data or voters’ list are easier to obtain from administrative headquarters. However, administrative boundaries do not always consist of homogenous group of people. Since it is desirable that a modest descriptive study does not cover a number of different groups of people, with widely differing ways of life or customs, it may be necessary to restrict the study to a particular ethnic group, and thus ensure better genetic or cultural homogeneity. Alternatively, a population may be defined in relation to a prominent geographic feature, such as a river, or mountain, which imposes a certain uniformity of ways of life, attitudes, and behavior upon the people who live in the vicinity.

If cases of a disease are being ascertained through their attendance at a hospital outpatient department (OPD), rather than by field surveys in the community, it will be necessary to define the population according to the so-called catchment area of the hospital OPD. For administrative purposes, a dispensary, health center or hospital is usually considered to serve a population within a defined geographic area. But these catchment areas may only represent in a crude manner with the actual use of medical facilities by the local people. For example, in OPD study of psychiatric illnesses in a particular hospital with a defined catchment area, many people with psychiatric illnesses may not visit the particular OPD and may seek treatment from traditional healers or religious leaders.

Catchment areas depend on the demography of the area and the accessibility of the health center or hospital. Accessibility has three dimensions – physical, economic and social.[ 2 ] Physical accessibility is the time required to travel to the health center or medical facility. It depends on the topography of the area (e.g. hill and tribal areas with poor roads have problems of physical accessibility). Economic accessibility is the paying capacity of the people for services. Poverty may limit health seeking behavior if the person cannot afford the bus fare to the health center even if the health services may be free of charge. It may also involve absence from work which, for daily wage earners, is a major economic disincentive. Social factors such as caste, culture, language, etc. may adversely affect accessibility to health facility if the treating physician is not conversant with the local language and customs. In such situations, the patient may feel more comfortable with traditional healers.

Ascertainment of a particular disease within a particular area may be incomplete either because some patient may seek treatment elsewhere or some patients do not seek treatment at all. Focus group discussions (qualitative study) with local people, especially those residing away from the health center, may give an indication whether serious underreporting is occurring.

When it is impossible to relate cases of a disease to a population, perhaps because the cases were ascertained through a hospital with an undefined catchment area, proportional morbidity rates may be used. These rates have been widely used in cancer epidemiology where the number of cases of one form of cancer is expressed as a proportion of the number of cases of all forms of cancer among patients attending the same hospital during the same period.

POPULATIONS IN ANALYTIC STUDIES

Case control studies.

As opposed to descriptive studies where a study population is defined and then observations are made on a representative sample from it, in case control studies observations are made on a group of patients. This is known as the study group , which usually is not selected by sampling of a defined larger group. For instance, a study on patients of bipolar disorder may include every patient with this disorder attending the psychiatry OPD during the study period. One should not forget, however, that in this situation also, there is a hypothetical population consisting of all patients with bipolar disorder in the universe (which may be a certain region, a country or globally depending on the extent of the generalization intended from the findings of the study). Case control studies are often carried out in hospital settings because this is more convenient and accessible group than cases in the community at large. However, the two groups of cases may differ in many respects. At the outset of the study, it should be deliberated whether these differences would affect the external validity (generalization) of the study. Usually, analytic studies are not carried out in groups containing atypical cases of the disorder, unless there is a special indication to do so.

Populations in cohort studies

Basically, cohort studies compare two groups of people (cohorts) and demonstrate whether or not there are more cases of the disease among the cohort exposed to the suspected cause than among the cohort not exposed. To determine whether an association exists between positive family history of schizophrenia and subsequent schizophrenia in persons having such a history, two cohorts would be required: first, the exposed group, that is, people with a family history of mental disorders (the suspected cause) and second, the unexposed group, that is, people without a family history of mental disorders. These two cohorts would need to be followed up for a number of years and cases of schizophrenia in either group would be recorded. If a positive family history is associated with development of schizophrenia, then more cases would occur in the first group than in the second group.

The crucial challenges in a cohort study are that it should include participants exposed to a particular cause being investigated and that it should consist of persons who can be followed up for the period of time between exposure (cause) and development of the disorder. It is vital that the follow-up of a cohort should be complete as far as possible. If more than a small proportion of persons in the cohort cannot be traced (loss to follow-up or attrition), the findings will be biased , in case these persons differ significantly from those remaining in the study.

Depending on the type of exposure being studied, there may or may not be a range of choice of cohort populations exposed to it who may form a larger population from which one has to select a study sample. For instance, if one is exploring association between occupational hazard such as job stress in health care workers in intensive care units (ICUs) and subsequent development of drug addiction, one has to, by the very nature of the research question, select health care workers working in ICUs. On the other hand, cause effect study for association between head injury and epilepsy offers a much wider range of possible cohorts.

Difficulties in making repeated observations on cohorts depend on the length of time of the study. In correlating maternal factors (pregnancy cohort) with birth weight, the period of observation is limited to 9 months. However, if in a study it is tried to find the association between maternal nutrition during pregnancy and subsequent school performance of the child, the study will extend to years. For such long duration investigations, it is wise to select study cohorts that are firstly, not likely to migrate, cooperative and likely to be so throughout the duration of the study, and most importantly, easily accessible to the investigator so that the expense and efforts are kept within reasonable limits. Occupational groups such as the armed forces, railways, police, and industrial workers are ideal for cohort studies. Future developments facilitating record linkage such as the Unique Identification Number Scheme may give a boost to cohort studies in the wider community.

A sample is any part of the fully defined population. A syringe full of blood drawn from the vein of a patient is a sample of all the blood in the patient's circulation at the moment. Similarly, 100 patients of schizophrenia in a clinical study is a sample of the population of schizophrenics, provided the sample is properly chosen and the inclusion and exclusion criteria are well defined.

To make accurate inferences, the sample has to be representative. A representative sample is one in which each and every member of the population has an equal and mutually exclusive chance of being selected.

Sample size

Inputs required for sample size calculation have been dealt from a clinical researcher's perspective avoiding the use of intimidating formulae and statistical jargon in an earlier issue of the journal.[ 1 ]

Target population, study population and study sample

A population is a complete set of people with a specialized set of characteristics, and a sample is a subset of the population. The usual criteria we use in defining population are geographic, for example, “the population of Uttar Pradesh”. In medical research, the criteria for population may be clinical, demographic and time related.

Clinical and demographic characteristics define the target population, the large set of people in the world to which the results of the study will be generalized (e.g. all schizophrenics).

The study population is the subset of the target population available for study (e.g. schizophrenics in the researcher's town).

The study sample is the sample chosen from the study population.

METHODS OF SAMPLING

Purposive (non-random samples).

Volunteers who agree to participate

Snowball sample, where one case identifies others of his kind (e.g. intravenous drug users)

Convenient sample such as captive medical students or other readily available groups

Quota sampling, at will selection of a fixed number from each group

Referred cases who may be under pressure to participate

Haphazard with combination of the above methods

Non-random samples have certain limitations. The larger group (target population) is difficult to identify. This may not be a limitation when generalization of results is not intended. The results would be valid for the sample itself (internal validity). They can, nevertheless, provide important clues for further studies based on random samples. Another limitation of non-random samples is that statistical inferences such as confidence intervals and tests of significance cannot be estimated from non-random samples. However, in some situations, the investigator has to make crucial judgments. One should remember that random samples are the means but representativeness is the goal. When non-random samples are representative (compare the socio-demographic characteristics of the sample subjects with the target population), generalization may be possible.

Random sampling methods

Simple random sampling.

A sample may be defined as random if every individual in the population being sampled has an equal likelihood of being included. Random sampling is the basis of all good sampling techniques and disallows any method of selection based on volunteering or the choice of groups of people known to be cooperative.[ 3 ]

In order to select a simple random sample from a population, it is first necessary to identify all individuals from whom the selection will be made. This is the sampling frame. In developing countries, listings of all persons living in an area are not usually available. Census may not catch nomadic population groups. Voters’ and taxpayers’ lists may be incomplete. Whether or not such deficiencies are major barriers in random sampling depends on the particular research question being investigated. To undertake a separate exercise of listing the population for the study may be time consuming and tedious. Two-stage sampling may make the task feasible.

The usual method of selecting a simple random sample from a listing of individuals is to assign a number to each individual and then select certain numbers by reference to random number tables which are published in standard statistical textbooks. Random number can also be generated by statistical software such as EPI INFO developed by WHO and CDC Atlanta.

Systematic sampling

A simple method of random sampling is to select a systematic sample in which every n th person is selected from a list or from other ordering. A systematic sample can be drawn from a queue of people or from patients ordered according to the time of their attendance at a clinic. Thus, a sample can be drawn without an initial listing of all the subjects. Because of this feasibility, a systematic sample may have some advantage over a simple random sample.

To fulfill the statistical criteria for a random sample, a systematic sample should be drawn from subjects who are randomly ordered. The starting point for selection should be randomly chosen. If every fifth person from a register is being chosen, then a random procedure must be used to determine whether the first, second, third, fourth, or fifth person should be chosen as the first member of the sample.

Multistage sampling

Sometimes, a strictly random sample may be difficult to obtain and it may be more feasible to draw the required number of subjects in a series of stages. For example, suppose we wish to estimate the number of CATSCAN examinations made of all patients entering a hospital in a given month in the state of Maharashtra. It would be quite tedious to devise a scheme which would allow the total population of patients to be directly sampled. However, it would be easier to list the districts of the state of Maharashtra and randomly draw a sample of these districts. Within this sample of districts, all the hospitals would then be listed by name, and a random sample of these can be drawn. Within each of these hospitals, a sample of the patients entering in the given month could be chosen randomly for observation and recording. Thus, by stages, we draw the required sample. If indicated, we can introduce some element of stratification at some stage (urban/rural, gender, age).

It should be cautioned that multistage sampling should only be resorted to when difficulties in simple random sampling are insurmountable. Those who take a simple random sample of 12 hospitals, and within each of these hospitals select a random sample of 10 patients, may believe they have selected 120 patients randomly from all the 12 hospitals. In statistical sense, they have in fact selected a sample of 12 rather than 120.[ 4 ]

Stratified sampling

If a condition is unevenly distributed in a population with respect to age, gender, or some other variable, it may be prudent to choose a stratified random sampling method. For example, to obtain a stratified random sample according to age, the study population can be divided into age groups such as 0–5, 6–10, 11–14, 15–20, 21–25, and so on, depending on the requirement. A different proportion of each group can then be selected as a subsample either by simple random sampling or systematic sampling. If the condition decreases with advancing age, then to include adequate number in the older age groups, one may select more numbers in older subsamples.

Cluster sampling

In many surveys, studies may be carried out on large populations which may be geographically quite dispersed. To obtain the required number of subjects for the study by a simple random sample method will require large costs and will be cumbersome. In such cases, clusters may be identified (e.g. households) and random samples of clusters will be included in the study; then, every member of the cluster will also be part of the study. This introduces two types of variations in the data – between clusters and within clusters – and this will have to be taken into account when analyzing data.

Cluster sampling may produce misleading results when the disease under study itself is distributed in a clustered fashion in an area. For example, suppose we are studying malaria in a population. Malaria incidence may be clustered in villages having stagnant water collections which may serve as a source of mosquito breeding. In villages without such water stagnation, there will be lesser malaria cases. The choice of few villages in cluster sampling may give erroneous results. The selection of villages as a cluster may be quite unrepresentative of the whole population by chance.[ 5 ]

Lot quality assurance sampling

Lot quality assurance sampling (LQAS), which originated in the manufacturing industry for quality control purposes, was used in the nineties to assess immunization coverage, estimate disease prevalence, and evaluate control measures and service coverage in different health programs.[ 6 ] Using only a small sample size, LQAS can effectively differentiate between areas that have or have not met the performance targets. Thus, this method is used not only to estimate the coverage of quality care but also to identify the exact subdivisions where it is deficient so that appropriate remedial measures can be implemented.

The choice of sampling methods is usually dictated by feasibility in terms of time and resources. Field research is quite messy and difficult like actual battle. It may be sometimes difficult to get a sample which is truly random. Most samples therefore tend to get biased. To estimate the magnitude of this bias, the researcher should have some idea about the population from which the sample is drawn. In conclusion, the following quote cited by Bradford Hill[ 4 ] elegantly sums up the benefit of random sampling:

…The actual practice of medicine is virtually confined to those members of the population who either are ill, or think they are ill, or are thought by somebody to be ill, and these so amply fill up the working day that in the course of time one comes unconsciously to believe that they are typical of the whole. This is not the case. The use of a random sample brings to light the individuals who are ill and know they are ill but have no intention of doing anything about it, as well as those who have never been ill, and probably never will be until their final illness. These would have been inaccessible to any other method of approach but that of the random sample… . J. H. Sheldon

Source of Support: Nil.

Conflict of Interest: None declared.

• 1. Banerjee A, Chaudhury S, Singh DK, Banerjee I, Mahato AK, Haldar S. Statistics without tears - inputs for sample size calculations. Indian Psychiatr Jr. 2007;16:150–2. [ Google Scholar ]
• 2. Barker DJ, Hall A J. Practical epidemiology. 4th ed. ELBS; 1994. pp. 30–43. [ Google Scholar ]
• 3. Indrayan A. Basic methods of medical research. India: AITBS Publishers; 2008. p. 116. [ Google Scholar ]
• 4. Hill AB, Hill ID. Principles of medical statistics. 12th ed. New Delhi: B I Publications Pvt Ltd; 1993. pp. 12–22. [ Google Scholar ]
• 5. WHO. 2nd ed. WHO Regional office of Western Pacific, Manila; 2001. Health Research Methodology; p. 82. [ Google Scholar ]
• 6. WHO. Vaccine research and development: Geneva; 1996. Monitoring immunization services using the lot quality technique.Global program for vaccines and immunization. [ Google Scholar ]
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Population and Target Population in Research Methodology

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